02599nam a22002177a 450000500170000000800410001702000180005802000180007604000410009404100080013506000110014324500560015425000110021026000380022130000450025949000330030450400530033750519210039065000410231170000290235220240912091245.0240912b           ||||| |||| 00| 0 eng d  a9781071639603  a9781071639610  aCO-BoUCMcDavid MorenodDavid Moreno  aeng  aWH 50610aBaculovirus bmethods and protocolscManon M.J. Cox  a1a. Ed  aNew York, NY bHumana Pressc2024  axv, 343 Paginas bilustraciones a color   aMethods in molecular biology  aIncluye referencias bibliográficas e índices  rExpansion and Storage of Insect Cells and Virus Stocks -- Construction of Recombinant Baculoviruses -- A Time and Cost-Effective Pipeline for Expression Screening and Protein Production in Insect Cells Based on the HR-Bac Toolbox to Generate Recombinant Baculoviruses -- Optimized Workflow from Gene to Product -- TOPO Cloning for Efficient Plasmid Construction -- Adaptive Laboratory Evolution to Improve Recombinant Protein Production Using Insect Cells -- RNAi-Mediated Silencing in the Insect Cell-Baculovirus Expression System -- Scarless Baculovirus Genome Editing Using Lambda Red Recombineering in E. coli -- Probing Baculovirus Vector Gene Essentiality for Foreign Gene Expression Using a CRISPR-Cas9 System -- Generation of Complex Protein Structures by Co-Infection with High Quality Recombinant Baculovirus -- Production of Secreted Antibody in Baculovirus Expression Vector System -- Production of Recombinant Viral Antigens Using the Baculovirus-Insect Cell Expression System -- Protein Production at the 100L Scale -- Recombinant AAV Production -- Recombinant AAV Purification -- Extraction of Modified Adeno-Associated Virus-Like Particles Displaying Peptides on Their Surface -- One-Step Purification Strategy for Cowpea Chlorotic Mottle Virus-Like Particles Produced by the IC-BEVS -- Purification Process for a Secreted Protein Produced in Insect Cells -- Functional Baculovirus Particle Quantification via Plaque Assay -- TCID50 Assay: A Simple Method to Determine Baculovirus Titer -- Rapid Two-Day Baculovirus Titering Using Flow Cytometry -- A Microsphere Immunoassay for the Quantitative Detection of Antigens in Cell Culture Supernatant -- Non-Viral Platform for Expression of Recombinant Protein in Insect Cells -- Assembly of Baculovirus Vectors for Multiplexed Prime Editing -- Recombinant Protein Production in Suspension Mammalian Cells Using the BacMam Baculovirus Expression System.  14aBaculovirusxManuales de laboratorio1 aCox, Manon M. J.eeditor