TY  - BOOK
AU  - Cox,Manon M.J.
TI  - Baculovirus : methods and protocols
T2  - Methods in molecular biology
SN  - 9781071639603
PY  - 2024///
CY  - New York, NY 
PB  - Humana Press
KW  - Baculovirus
KW  - Manuales de laboratorio
N1  - Incluye referencias bibliográficas e índices; Expansion and Storage of Insect Cells and Virus Stocks -- Construction of Recombinant Baculoviruses -- A Time and Cost-Effective Pipeline for Expression Screening and Protein Production in Insect Cells Based on the HR-Bac Toolbox to Generate Recombinant Baculoviruses -- Optimized Workflow from Gene to Product -- TOPO Cloning for Efficient Plasmid Construction -- Adaptive Laboratory Evolution to Improve Recombinant Protein Production Using Insect Cells -- RNAi-Mediated Silencing in the Insect Cell-Baculovirus Expression System -- Scarless Baculovirus Genome Editing Using Lambda Red Recombineering in E. coli -- Probing Baculovirus Vector Gene Essentiality for Foreign Gene Expression Using a CRISPR-Cas9 System -- Generation of Complex Protein Structures by Co-Infection with High Quality Recombinant Baculovirus -- Production of Secreted Antibody in Baculovirus Expression Vector System -- Production of Recombinant Viral Antigens Using the Baculovirus-Insect Cell Expression System -- Protein Production at the 100L Scale -- Recombinant AAV Production -- Recombinant AAV Purification -- Extraction of Modified Adeno-Associated Virus-Like Particles Displaying Peptides on Their Surface -- One-Step Purification Strategy for Cowpea Chlorotic Mottle Virus-Like Particles Produced by the IC-BEVS -- Purification Process for a Secreted Protein Produced in Insect Cells -- Functional Baculovirus Particle Quantification via Plaque Assay -- TCID50 Assay: A Simple Method to Determine Baculovirus Titer -- Rapid Two-Day Baculovirus Titering Using Flow Cytometry -- A Microsphere Immunoassay for the Quantitative Detection of Antigens in Cell Culture Supernatant -- Non-Viral Platform for Expression of Recombinant Protein in Insect Cells -- Assembly of Baculovirus Vectors for Multiplexed Prime Editing -- Recombinant Protein Production in Suspension Mammalian Cells Using the BacMam Baculovirus Expression System.
ER  -